Thin layer chromatography (TLC) is a method in which a suitable stationary phase is coated on a glass plate, plastic or aluminum substrate to form a uniform thin layer. After the sample is sampled and expanded, the specific shift value (RF) of the chromatogram obtained by the same method is compared with that of the appropriate reference substance according to the specific shift value (RF), which is used for the identification of drugs, impurity inspection or content determination. Thin layer chromatography (TLC) is an important experimental technique for rapid separation and qualitative analysis of small amounts of substances. It is also used to track the reaction process.
High performance liquid chromatography (HPLC) is an important branch of chromatography. It uses liquid as the mobile phase and adopts high-pressure infusion system to pump single solvent with different polarity or mixed solvent with different proportion, buffer solution and other mobile phases into the chromatographic column with fixed phase. After each component in the column is separated, it enters the detector for detection, so as to realize the analysis of samples.
